Detection of the lipid-linked precursor oligosaccharide of N-linked protein glycosylation in Drosophila melanogaster

FEBS Lett. 1991 Sep 23;290(1-2):58-60. doi: 10.1016/0014-5793(91)81225-w.

Abstract

The presence of a glycan of the same molecular size as the lipid linked precursor oligosaccharide (Glc3Man9GlcNAc2) of the N-linked protein glycosylation pathway in mammalian cells has been detected in a glycolipid fraction of cultured Drosophila melanogaster cells. Oligosaccharide sequencing studies were consistent with the existence of a glucosylated high mannose containing structure, which may be the common precursor for N-linked protein glycosylation in insect cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Drosophila melanogaster / metabolism*
  • Glycolipids / chemistry
  • Glycolipids / metabolism*
  • Glycoproteins / metabolism*
  • Glycosylation
  • Oligosaccharides / chemistry
  • Oligosaccharides / metabolism
  • Protein Processing, Post-Translational*

Substances

  • Glycolipids
  • Glycoproteins
  • Oligosaccharides