Abstract
Background:
Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection.
Results:
Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces.
Conclusions:
PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker.
© Springer Science+Business Media, LLC 2009
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antigens, Neoplasm / genetics
-
Antigens, Neoplasm / metabolism*
-
Bacterial Translocation
-
Biomarkers / metabolism
-
Biomarkers, Tumor / genetics
-
Biomarkers, Tumor / metabolism*
-
Calcium, Dietary / metabolism
-
Disease Models, Animal
-
Eating
-
Enterocytes / metabolism
-
Enterocytes / microbiology
-
Feces / chemistry*
-
Feces / microbiology
-
Ileitis / metabolism
-
Ileitis / microbiology
-
Ileum / metabolism*
-
Ileum / microbiology
-
Intestinal Mucosa / metabolism
-
Intestinal Mucosa / microbiology
-
Lectins, C-Type / genetics
-
Lectins, C-Type / metabolism*
-
Male
-
Pancreatitis-Associated Proteins
-
RNA, Messenger / metabolism
-
Rats
-
Rats, Wistar
-
Salmonella Infections, Animal / metabolism*
-
Salmonella Infections, Animal / microbiology
-
Salmonella enteritidis / pathogenicity*
-
Severity of Illness Index
-
Time Factors
Substances
-
Antigens, Neoplasm
-
Biomarkers
-
Biomarkers, Tumor
-
Calcium, Dietary
-
Lectins, C-Type
-
Pancreatitis-Associated Proteins
-
REG3A protein, human
-
RNA, Messenger
-
Reg3b protein, rat