Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana

Gene. 1991 Aug 15;104(2):133-8. doi: 10.1016/0378-1119(91)90242-4.

Abstract

Both cDNA and genomic clones for the nuclear genes encoding chloroplast (cp) (gapA and gapB) and cytosolic (gapC) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Arabidopsis thaliana have been isolated and characterized. Genomic Southern-blot analyses indicate that there is only one copy of each gapA, gapB and gapC gene in A. thaliana. Comparison of the deduced amino acid (aa) sequences shows that the A and B subunits are highly similar (80% positional aa identity), while there is less similarity between the cp and cytosolic subunits (45% aa identity). These relationships are consistent with the idea that the cp and cytosolic GAPDHs evolved from different lineages, as suggested in our previous study of tobacco GAPDHs [Shih et al., Cell 47 (1986) 73-80]. In addition, the chromosomal locations for the three gap genes were determined by restriction fragment length polymorphism mapping; the three gap genes are not closely linked, gapA (55.8 cM) and gapC (0.0 cM) are on chromosome 3, and gapB (51.3 cM) is on chromosome 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Nucleus
  • Chloroplasts / metabolism*
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytosol / metabolism*
  • DNA
  • Genomic Library
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Molecular Sequence Data
  • Peptide Fragments / genetics*
  • Peptide Fragments / metabolism
  • Plants / genetics
  • Sequence Homology, Nucleic Acid

Substances

  • Peptide Fragments
  • glyceraldehyde 3-phosphate dehydrogenase (304-313)
  • DNA
  • Glyceraldehyde-3-Phosphate Dehydrogenases

Associated data

  • GENBANK/M64114
  • GENBANK/M64115
  • GENBANK/M64116
  • GENBANK/M64117
  • GENBANK/M64118
  • GENBANK/M64119
  • GENBANK/S54966
  • GENBANK/S54968
  • GENBANK/S56946
  • GENBANK/S56949
  • GENBANK/S56951
  • GENBANK/S60812
  • GENBANK/X56411