Nitric oxide (NO) detection is a critical issue for environmental safety and medical diagnosis due reasons with regard to the toxic properties for human body and metabolic index for respiratory disease, respectively. Development of gas sensor with high sensitivity is very important because the concentration of NO gas in the environment and respiratory tract is extremely low, therefore not readily detectable. The material with nanostructure can improve the sensitivity of sensor owning to surface effect and size effect. Herein, we developed a new type of gaseous nanosensor assembled by 34 nanowires of conducting polymer, PEDOT: PSS. The nanowires were fabricated by dip pen nanolithography (DPN) with the length of 55 um and diameter of 300 nm between golden wires. The NO gas measurement is based on chemiresistor based methods. The result of dynamic measurement of NO gas at 100 ppm shows repeatability and stability; the recovery time is 10.4 minutes. Moreover, the lowest concentration of NO gas in static measurement is 10ppm at 80 C, which also shows the ability sensing at low temperature.