Quantitative magnetization transfer measured pool-size ratio reflects optic nerve myelin content in ex vivo mice

Magn Reson Med. 2009 Feb;61(2):364-71. doi: 10.1002/mrm.21850.


Optic nerves from mice that have undergone retinal ischemia were examined using a newly implemented quantitative magnetization transfer (qMT) technique. Previously published results indicate that the optic nerve from retinal ischemia mice suffered significant axon degeneration without detectable myelin injury at 3 days after reperfusion. At this time point, we acquired ex vivo qMT parameters from both shiverer mice (which have nearly no myelin) and control mice that have undergone retinal ischemia, and these qMT measures were compared with diffusion tensor imaging (DTI) results. Our findings suggests that the qMT estimated ratio of the pool sizes of the macromolecular and free water protons reflected the different myelin contents in the optic nerves between the shiverer and control mice. This pool size ratio was specific to myelin content only and was not significantly affected by the presence of axon injury in mouse optic nerve 3 days after retinal ischemia.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Diffusion Magnetic Resonance Imaging / methods*
  • Image Interpretation, Computer-Assisted / methods*
  • Ischemia / pathology*
  • Mice
  • Nerve Fibers, Myelinated / pathology*
  • Optic Nerve / pathology*
  • Retinal Artery / pathology*