In vitro priming to direct neuronal fate in adult neural progenitor cells

Exp Neurol. 2009 Apr;216(2):520-4. doi: 10.1016/j.expneurol.2008.12.023. Epub 2009 Jan 7.


We have previously reported methods that allow for the routine isolation, culture and transplantation of multipotent neural progenitor cells (NPCs) derived from the adult rat sub-ventricular zone (SVZ). In order to expand these techniques, the aim of the current study was to develop an in vitro strategy which can "prime" proliferating adult NPCs towards a neuronal fate during their subsequent differentiation under standard conditions. Our results show that transient exposure to lithium chloride during in vitro proliferation of adult SVZ-derived NPCs has the ability to alter differential fate in vitro and increase the proportion of cells expressing neuronal markers while at the same time causing a reduction in glial progeny. Treatment of adult NPCs with lithium chloride may therefore provide a novel mechanism by which adult NPCs can be primed towards a specific neuronal fate for cell replacement therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology
  • Adult Stem Cells / drug effects
  • Adult Stem Cells / physiology*
  • Analysis of Variance
  • Animals
  • Antineoplastic Agents / pharmacology
  • Bromodeoxyuridine / metabolism
  • Calbindins
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology*
  • Cell Survival
  • Cells, Cultured
  • Lithium Chloride / pharmacology
  • Male
  • Nerve Tissue Proteins / pharmacology
  • Neurons / drug effects
  • Neurons / physiology*
  • Rats
  • Rats, Wistar
  • S100 Calcium Binding Protein G / pharmacology
  • Time Factors
  • Tretinoin / pharmacology


  • Adjuvants, Immunologic
  • Antineoplastic Agents
  • Calbindins
  • Nerve Tissue Proteins
  • S100 Calcium Binding Protein G
  • Tretinoin
  • Bromodeoxyuridine
  • Lithium Chloride