KITENIN increases invasion and migration of mouse squamous cancer cells and promotes pulmonary metastasis in a mouse squamous tumor model

FEBS Lett. 2009 Feb 18;583(4):711-7. doi: 10.1016/j.febslet.2009.01.014. Epub 2009 Jan 21.


KAI1 C-terminal interacting tetraspanin (KITENIN) is reported to promote metastasis in mouse colon cancer models. We investigated the role of KITENIN on the progression of squamous cell carcinoma (SCC). In a preliminary clinical study using resected tissues from head and neck SCC patients, KITENIN was highly expressed in tumors and metastatic lymph nodes, while KAI1 was more increased in adjacent mucosa than in tumor. KITENIN-transfected mouse squamous cancer (SCC VII/KITENIN) cells showed significantly higher invasion, migration, and proliferation than empty vector-transfected cells. In syngeneic mouse squamous tumor models, more increased tumor volume and enhanced lung metastasis were found in SCC VII/KITENIN cells-injected mice. Thus, KITENIN increases invasion and migration of squamous cancer cells and thereby promotes distant metastasis in mouse squamous tumor models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / secondary*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line, Tumor
  • Culture Media, Serum-Free
  • Disease Models, Animal*
  • Female
  • Genetic Vectors
  • Head and Neck Neoplasms / genetics
  • Head and Neck Neoplasms / secondary*
  • Humans
  • Lung Neoplasms / genetics
  • Lung Neoplasms / secondary*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Inbred C3H
  • Neoplasm Invasiveness / genetics
  • Neoplasm Metastasis
  • RNA, Messenger / metabolism
  • Transfection
  • Xenograft Model Antitumor Assays


  • Carrier Proteins
  • Culture Media, Serum-Free
  • Membrane Proteins
  • RNA, Messenger
  • VANGL1 protein, human