Red Blood Cell Glutathione Peroxidase Activity in Female Nulligravid and Pregnant Rats

Reprod Biol Endocrinol. 2009 Jan 26;7:7. doi: 10.1186/1477-7827-7-7.


Background: The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise.

Methods: Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes.

Results: The optimization, when applied to age paired rats, both nulligravid and pregnant, shows that pregnancy induces, at seventeen d of pregnancy, an increase of both reactive oxygen substance concentration in red blood cells and membrane glutathione peroxidase activity.

Conclusion: The glutathione peroxidase increase in erythrocyte membranes is induced by systemic oxidative stress long lasting rat pregnancy.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Erythrocyte Membrane / enzymology*
  • Female
  • Glutathione Peroxidase / blood*
  • Glutathione Peroxidase / chemistry
  • Gravidity
  • Kinetics
  • Models, Molecular
  • Oxidative Stress
  • Pregnancy / blood*
  • Pregnancy / metabolism
  • Rats
  • Rats, Wistar
  • Spectrophotometry / methods*


  • Glutathione Peroxidase