Engineering a function into a glycosyltransferase

Chem Biol. 2009 Jan 30;16(1):28-35. doi: 10.1016/j.chembiol.2008.12.003.

Abstract

As glycosyltransferases found in nature often show distinct substrate specificity, glycosyltransferase engineering is an important research field. In this work, we were able to introduce an activity into a glycosyltransferase involved in natural product (landomycin E) biosynthesis. This was achieved by recognizing hot spot amino acids in glycosyltransferases which are strongly involved in determining substrate specificity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aminoglycosides / biosynthesis
  • Glycosyltransferases / genetics*
  • Glycosyltransferases / metabolism
  • Molecular Sequence Data
  • Mutation
  • Protein Engineering
  • Sequence Homology, Amino Acid
  • Substrate Specificity

Substances

  • Aminoglycosides
  • landomycin E
  • Glycosyltransferases