Nicotinamide and its metabolite N-methylnicotinamide increase skin vascular permeability in rats

Clin Exp Dermatol. 2009 Apr;34(3):380-4. doi: 10.1111/j.1365-2230.2008.02922.x. Epub 2008 Oct 29.

Abstract

Background: It has been suggested that topically applied nicotinamide and its metabolite N-methylnicotinamide (NMN(+)) might be useful agents for treatment of dermatological disorders such as acne vulgaris and rosacea.

Aim: This study aimed to find out if the mechanism of these therapeutic effects depends on their vascular effects, by investigating if nicotinamide and NMN(+) are able to influence vascular permeability of the vessels in the skin on the back of Wistar rats.

Methods and results: A dose-dependent increase in vascular permeability was seen in rats treated intradermally with nicotinamide and NMN(+). Interestingly, a significantly stronger effect of NMN(+) compared with nicotinamide was evident. Increased vascular permeability in rats treated with 0.5% NMN(+) ointment was seen. Moreover, indomethacin, a cyclo-oxygenase 1 and 2 inhibitor and N(G)-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthase inhibitor, reduced the observed effects of nicotinamide and NMN(+).

Conclusions: This study provides direct in vivo evidence that nicotinamide and its metabolite NMN(+) increase skin vascular permeability in rats by a mechanism that may involve NO and prostaglandins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capillary Permeability / drug effects*
  • Capillary Permeability / physiology
  • Dose-Response Relationship, Drug
  • Injections, Intradermal
  • Male
  • Niacinamide / analogs & derivatives*
  • Niacinamide / pharmacology*
  • Nitric Oxide / physiology
  • Prostaglandins / physiology
  • Rats
  • Rats, Wistar
  • Skin / blood supply*
  • Skin / drug effects
  • Vitamin B Complex / pharmacology*

Substances

  • Prostaglandins
  • Vitamin B Complex
  • Niacinamide
  • Nitric Oxide
  • N-methylnicotinamide