High-performance liquid chromatographic determination, with ultraviolet detection, of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat tissues and simultaneously of normetanephrine and metanephrine for phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities

J Chromatogr. 1991 Jun 14;567(1):254-60. doi: 10.1016/0378-4347(91)80329-b.


A high-performance liquid chromatographic method, with ultraviolet detection, for the determination of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, and simultaneously of normetanephrine and metanephrine, is presented. The separation was carried out by reversed-phase ion-pair isocratic chromatography. This procedure was applied to the study of the content of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine in rat brain and adrenal glands and to the measurement of phenylethanolamine-N-methyltransferase or catechol-O-methyltransferase activities in rat adrenal gland.

MeSH terms

  • Adrenal Glands / chemistry
  • Animals
  • Brain Chemistry
  • Catechol O-Methyltransferase / metabolism*
  • Chromatography, High Pressure Liquid
  • Male
  • Metanephrine / metabolism*
  • Normetanephrine / metabolism*
  • Phenylethanolamine N-Methyltransferase / metabolism*
  • Rats
  • S-Adenosylhomocysteine / metabolism*
  • S-Adenosylmethionine / metabolism*
  • Spectrophotometry, Ultraviolet


  • Normetanephrine
  • Metanephrine
  • S-Adenosylmethionine
  • S-Adenosylhomocysteine
  • Phenylethanolamine N-Methyltransferase
  • Catechol O-Methyltransferase