Cumulative effects of consecutive running sessions on hemolysis, inflammation and hepcidin activity

Eur J Appl Physiol. 2009 May;106(1):51-9. doi: 10.1007/s00421-009-0988-7. Epub 2009 Jan 31.


The effect of two running sessions completed within a 12-h period on hemolysis, inflammation, and hepcidin activity in endurance athletes was investigated. Ten males completed two experimental trials in a randomized, counterbalanced order. The two trials included (a) a one-running-session trial (T1) including 10 x 1 km interval repeats (90% peak VO2 velocity), and (b) a two-running-session trial (T2), comprising a continuous 10-km run (70% peak VO2 velocity), and a 10 x 1 km interval run (90% peak VO2 velocity) completed 12 h later. Interleukin-6 (IL-6), free hemoglobin (Hb), haptoglobin (Hp), iron, ferritin, and hepcidin were assessed post-exercise. After the T1 and T2 interval runs, free Hb was significantly increased and Hp significantly decreased (p <or= 0.05), with a cumulative effect shown in T2 after the second run (p <or= 0.05). The IL-6, serum iron, ferritin, and hepcidin activity were increased after each running session (p <or= 0.05), with no cumulative effect in T2. In conclusion, a cumulative effect of two running sessions on hemolysis was shown, but no similar effect with inflammation and hepcidin activity was evident.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antimicrobial Cationic Peptides / metabolism*
  • Antimicrobial Cationic Peptides / urine
  • Cross-Over Studies
  • Exercise Test
  • Ferritins / blood
  • Haptoglobins / analysis
  • Hemoglobins / analysis
  • Hemolysis / physiology*
  • Hepcidins
  • Humans
  • Inflammation* / blood
  • Inflammation* / urine
  • Interleukin-6 / blood
  • Iron / blood
  • Male
  • Physical Exertion / physiology
  • Running / physiology*
  • Time Factors


  • Antimicrobial Cationic Peptides
  • HAMP protein, human
  • Haptoglobins
  • Hemoglobins
  • Hepcidins
  • Interleukin-6
  • Ferritins
  • Iron