Comprehensive expression analysis of all Wnt genes and their major secreted antagonists during mouse limb development and cartilage differentiation

Gene Expr Patterns. 2009 Apr;9(4):215-23. doi: 10.1016/j.gep.2008.12.009. Epub 2009 Jan 13.

Abstract

Wnt signalling plays important roles in patterning and outgrowth of the vertebrate limb. Different mutations in Wnt genes, their antagonists or (co-)receptors result in patterning and outgrowth defects as well as chondrocyte and bone phenotypes in mouse and human. Understanding Wnt activity during mouse limb development and chondrogenesis requires a temporal and spatial overview of Wnt signalling key factor expression. Here we present a comparative expression analysis of all 19 Wnt genes and their major secreted antagonists of the Dickkopf (Dkk), Wisp and the secreted frizzled related protein (Sfrp) families during mouse limb development. Our study reveals new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. We also identified novel expression domains for the Wnt antagonists Sfrp1, Sfrp3, Sfrp5, Wisp1 as well as Dkk2 and Dkk3. We provide a full expression pattern for Wif1 in limb development, for which no limb expression had been documented so far.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CCN Intercellular Signaling Proteins
  • Cartilage / embryology
  • Cartilage / metabolism*
  • Chondrocytes / metabolism
  • Ectoderm / metabolism
  • Embryo, Mammalian / embryology
  • Embryo, Mammalian / metabolism
  • Extracellular Matrix Proteins / genetics
  • Forelimb / embryology
  • Forelimb / metabolism*
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental*
  • Glycoproteins / genetics
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / genetics
  • Mesoderm / metabolism
  • Mice / embryology
  • Mice / genetics*
  • Oncogene Proteins / genetics
  • Proto-Oncogene Proteins
  • Time Factors
  • Wnt Proteins / genetics*
  • Wnt2 Protein

Substances

  • CCN Intercellular Signaling Proteins
  • CCN4 protein, mouse
  • Dkk2 protein, mouse
  • Dkk3 protein, mouse
  • Extracellular Matrix Proteins
  • Glycoproteins
  • Intercellular Signaling Peptides and Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • Sfrp1 protein, mouse
  • Sfrp5 protein, mouse
  • WD repeat containing planar cell polarity effector
  • Wif1 protein, mouse
  • Wnt Proteins
  • Wnt2 Protein