Polyketide synthase thioesterases catalyze rapid hydrolysis of peptidyl thioesters

Bioorg Med Chem Lett. 2009 Mar 1;19(5):1413-5. doi: 10.1016/j.bmcl.2009.01.040. Epub 2009 Jan 19.


Polyketide synthase (PKS) thioesterases (TEs) catalyze the macrocyclization of linear acyl chains into macrolactones. Herein we show that peptide based substrates are processed by PKS TEs with greater catalytic efficiency than more native like acyl substrates. This result strengths the link between PKS and non-ribosomal peptide synthetase systems and provides a new tool for studying PKS TEs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Esterification
  • Esters
  • Hydrolysis
  • Peptides / metabolism*
  • Polyketide Synthases / metabolism*


  • Esters
  • Peptides
  • Polyketide Synthases