Consequences of perinatal treatment with L-arginine and antioxidants for the renal transcriptome in spontaneously hypertensive rats

Pflugers Arch. 2009 Jul;458(3):513-24. doi: 10.1007/s00424-009-0639-1. Epub 2009 Feb 3.


Treating spontaneously hypertensive rats (SHR) with L-arginine, taurine, and vitamins C and E (ATCE) during nephrogenesis (2 weeks before to 4 weeks after birth) persistently lowers blood pressure. Hypothetically, differential gene expression in kidney of SHR vs. normotensive Wistar-Kyoto rats (WKY) is partially corrected by maternal ATCE in SHR. Differential gene expression in 2-days, 2-weeks, and 48-week-old rats was studied using oligonucleotide chips. Transcription factor binding sites (TFBS) of differentially expressed genes were analyzed in silico. Differential gene expression varied between SHR+ATCE and SHR, suggesting both direct and indirect effects; but, few genes were modulated toward WKY level and there was little overlap between ages. TFBS analysis suggests less Elk-1-driven gene transcription in both WKY and SHR+ATCE vs. SHR at 2 days and 2 weeks. Concluding, in SHR, persistent antihypertensive effects of maternal ATCE are not primarily due to persistent corrective transcription. Less Elk-1-driven transcription at 2 days and 2 weeks may be involved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage*
  • Arginine / administration & dosage*
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Developmental / drug effects*
  • Hypertension / metabolism*
  • Kidney / drug effects
  • Kidney / metabolism*
  • Proteome / metabolism*
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Transcription Factors / metabolism*


  • Antioxidants
  • Proteome
  • Transcription Factors
  • Arginine