Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. Nov-Dec 2008;28(6A):3557-66.

The Impact of Enzymatic Degradation on the Uptake of Differently Sized Therapeutic Molecules

Affiliations
  • PMID: 19189635
Free article

The Impact of Enzymatic Degradation on the Uptake of Differently Sized Therapeutic Molecules

Arne Erikson et al. Anticancer Res. .
Free article

Abstract

Background: The extracellular matrix represents a major barrier for drug delivery. This work compares the effects of collagenase and hyaluronidase on tumour uptake and distribution of two differently sized therapeutic molecules, IgG and liposomal doxorubicin.

Materials and methods: The enzymes were injected i.v. prior to the therapeutic molecules, and uptake and distribution were studied by confocal laser scanning microscopy. The therapeutic molecules were colocalized with the vasculature and collagen network visualized by the second harmonic signal.

Results: Hyaluronidase increased the uptake of liposomal doxorubicin to a small extent, whereas collagenase had no effect. Collagenase increased, but hyaluronidase reduced the uptake of IgG. Neither of the enzymes induced changes in the collagen network measured by the second harmonic signal.

Conclusion: Degradation of the collagen network improves delivery of molecules with the size of IgG, whereas degradation of the gel of glycosaminoglycans has a higher impact on the distribution of small drugs such as doxorubicin.

Similar articles

See all similar articles

Cited by 13 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback