De Novo identification of single nucleotide mutations in Caenorhabditis elegans using array comparative genomic hybridization

Jason S Maydan; H Mark Okada; Stephane Flibotte; Mark L Edgley; Donald G Moerman

doi:10.1534/genetics.108.100065

De Novo identification of single nucleotide mutations in Caenorhabditis elegans using array comparative genomic hybridization

Genetics. 2009 Apr;181(4):1673-7. doi: 10.1534/genetics.108.100065. Epub 2009 Feb 2.

Authors

Jason S Maydan¹, H Mark Okada, Stephane Flibotte, Mark L Edgley, Donald G Moerman

Affiliation

¹ Department of Zoology, University of British Columbia V6T 1Z4, Vancouver, British Columbia, Canada.

Abstract

Array comparative genomic hybridization (aCGH) has been used primarily to detect copy-number variants between two genomes. Here we report using aCGH to detect single nucleotide mutations on oligonucleotide microarrays with overlapping 50-mer probes. This technique represents a powerful method for rapidly detecting novel homozygous single nucleotide mutations in any organism with a sequenced reference genome.

Publication types

Evaluation Study
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Animals
Caenorhabditis elegans / genetics*
Comparative Genomic Hybridization / methods*
Databases, Genetic
Genome, Helminth
Genomics / methods
Oligonucleotide Array Sequence Analysis / methods*
Point Mutation
Polymorphism, Single Nucleotide*
Software