Methylation status of RASSF1A in patients with chronic myeloid leukemia

Leuk Res. 2009 Aug;33(8):1130-2. doi: 10.1016/j.leukres.2009.01.003. Epub 2009 Feb 3.


RASSF1A, a key cell cycle related gene, is expressed in all hematopoietic cells, it is implicated in ras signaling pathway and its promoter hypermethylation is observed in a wide variety of solid tumors. Till now, RASSF1A methylation status has not been investigated in patients with chronic myeloid leukemia (CML). In this study, we analyzed 41 patients carrying the BCR-ABL rearrangement, in different stages of the disease. No patient displayed RASSF1A promoter methylation, although the K562 erythroleukemia cell line, bearing the BCR-ABL rearrangement, was found methylated. Thus, our findings indicate that RASSF1A methylation does not appear to represent a critical step in the pathogenesis and/or the progression of CML.

MeSH terms

  • DNA Methylation*
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / metabolism*
  • Female
  • Gene Expression Regulation, Leukemic*
  • Genes, abl / genetics
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • K562 Cells
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism*
  • Male
  • Promoter Regions, Genetic*
  • Translocation, Genetic / genetics
  • Tumor Suppressor Proteins / biosynthesis*
  • Tumor Suppressor Proteins / genetics


  • DNA, Neoplasm
  • RASSF1 protein, human
  • Tumor Suppressor Proteins