Novel method for determination of substance P levels in unextracted human plasma by using acidification

Clin Vaccine Immunol. 2009 Apr;16(4):594-6. doi: 10.1128/CVI.00406-08. Epub 2009 Feb 4.


Substance P (SP) is a member of the tachykinin family and has an important role in immune responses. SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Hydrogen-Ion Concentration
  • Plasma / chemistry*
  • Substance P / analysis*


  • Substance P