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, 72 ( Pt 10), 2563-7

Characterization of the VPg-dsRNA Linkage of Infectious Pancreatic Necrosis Virus

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Characterization of the VPg-dsRNA Linkage of Infectious Pancreatic Necrosis Virus

J G Calvert et al. J Gen Virol.

Abstract

By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of 125I-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VP1 (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5' GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses.

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