Hydrophilic fluorescent nanogel thermometer for intracellular thermometry

J Am Chem Soc. 2009 Mar 4;131(8):2766-7. doi: 10.1021/ja807714j.


The first methodology to measure intracellular temperature is described. A highly hydrophilic fluorescent nanogel thermometer developed for this purpose stays in the cytoplasm and emits stronger fluorescence at a higher temperature. Thus, intracellular temperature variations associated with biological processes can be monitored by this novel thermometer with a temperature resolution of better than 0.5 degrees C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry*
  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Cytological Techniques / instrumentation*
  • Cytological Techniques / methods
  • Fluorescent Dyes / chemistry*
  • Nanostructures / chemistry*
  • Thermometers*


  • Acrylic Resins
  • Fluorescent Dyes
  • poly-N-isopropylacrylamide