A single component two-valent LcrV-F1 vaccine protects non-human primates against pneumonic plague

Vaccine. 2009 May 26;27(25-26):3471-4. doi: 10.1016/j.vaccine.2009.01.050. Epub 2009 Feb 5.

Abstract

Yersinia pestis continues to pose a threat as a potential biological weapon and is recognized by public health experts as a re-emerging infectious disease. Therefore there is great interest in developing a safe and effective vaccine. Vaccines against plague containing both the Fraction 1 (F1) and V antigens of Y. pestis have shown promise in protecting animal models against pneumonic plague, the deadliest form of the disease. Here we report on a plague vaccine consisting of the F1 and LcrV antigens fused to a single carrier molecule, the thermostable enzyme lichenase from Clostridium thermocellum, and expressed in and purified from Nicotiana benthamiana plants. When administered to Cynomolgus Macaques this purified plant-produced vaccine induced high titers of serum IgG, mainly of the IgG1 isotype, against both F1 and LcrV. These immunized animals were subsequently challenged and the LcrV-F1 plant-produced vaccine conferred complete protection against aerosolized Y. pestis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Female
  • Macaca fascicularis
  • Plague / prevention & control
  • Plague Vaccine / immunology*
  • Pore Forming Cytotoxic Proteins / genetics
  • Pore Forming Cytotoxic Proteins / immunology*
  • Recombinant Fusion Proteins / immunology*
  • Tobacco / genetics*
  • Vaccines, Synthetic / immunology*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • LcrV protein, Yersinia
  • Plague Vaccine
  • Pore Forming Cytotoxic Proteins
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • caf1 protein, Yersinia pestis