Crystallization and preliminary X-ray data of bromoperoxidase from Streptomyces aureofaciens ATCC 10762

H Sobek; T Haag; O Pfeifer; D Schomburg; F Lingens; K H van Pée

doi:10.1016/0022-2836(91)80199-5

Crystallization and preliminary X-ray data of bromoperoxidase from Streptomyces aureofaciens ATCC 10762

J Mol Biol. 1991 Sep 5;221(1):35-7. doi: 10.1016/0022-2836(91)80199-5.

Authors

H Sobek¹, T Haag, O Pfeifer, D Schomburg, F Lingens, K H van Pée

Affiliation

¹ Department of Molecular Structure Research, GBF-Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany.

PMID: 1920414
DOI: 10.1016/0022-2836(91)80199-5

Abstract

Bromoperoxidase from Streptomyces aureofaciens ATCC 10762, a non-haem haloperoxidase, has been crystallized using the hanging drop method. Preliminary X-ray diffraction studies show that the crystals belong to the cubic space group P2(1)3 with a = 123.4 A. The asymmetric unit contains a dimer of Mr = 60,200. The crystals diffract to at least 2.3 A resolution and are suitable for crystallographic structure analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Peroxidases / chemistry*
Streptomyces aureofaciens / enzymology*
X-Ray Diffraction

Substances

Peroxidases
bromide peroxidase