Myb transcription factors have been implicated in a wide variety of plant-specific processes, including secondary metabolism, cell shape determination, cell differentiation, and stress responses. Very recently, AtMyb41 from Arabidopsis (Arabidopsis thaliana) was described as a gene transcriptionally regulated in response to salinity, desiccation, cold, and abscisic acid. The corresponding transcription factor was suggested to control stress responses linked to cell wall modifications. In this work, we have characterized AtMyb41 further by subjecting independent AtMyb41-overexpressing lines to detailed transcriptome and metabolome analysis. Our molecular data indicate that AtMyb41 is involved in distinct cellular processes, including control of primary metabolism and negative regulation of short-term transcriptional responses to osmotic stress.