Effect of Tai Chi exercise on DNA damage, antioxidant enzymes, and oxidative stress in middle-age adults

J Phys Act Health. 2009 Jan;6(1):43-54. doi: 10.1123/jpah.6.1.43.


Background: The biochemical mechanisms involving oxidative stress to explain the relationship between exercise and healthy aging are still unclear.

Methods: Tai Chi participants and matched sedentary volunteers age 45 and above were enrolled. Glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) activities; levels of DNA damage using the comet assay; and malondialdehyde (MDA) and advanced glycation end products (AGE) were determined at 0, 6, and 12 months.

Results: Tai Chi subjects had decreased normal and increased mildly damaged DNA with elevated GPx activity after 6 months (n=25). Plasma MDA and AGE concentrations decreased significantly after 12 months (n=15) accompanied by increased SOD activity. This may be attributed to the hormesis effect, whereby mild induction of oxidative stress at the first 6 months of exercise resulted in stimulation of antioxidant defenses. These parameters were unchanged in the sedentary subjects in the first 6 months (n=27) except for elevated SOD activity. After 12 months, the sedentary subjects (n=17) had decreased normal DNA and increased severely damaged DNA with unaltered MDA and AGE levels while SOD and GPx activities were significantly elevated.

Conclusion: Regular Tai Chi exercise stimulated endogenous antioxidant enzymes and reduced oxidative damage markers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Analysis of Variance
  • Case-Control Studies
  • Catalase / blood
  • Comet Assay
  • DNA Damage*
  • Female
  • Glutathione Peroxidase / blood
  • Glycation End Products, Advanced / blood
  • Humans
  • Male
  • Malondialdehyde / blood
  • Middle Aged
  • Oxidative Stress*
  • Statistics, Nonparametric
  • Superoxide Dismutase / blood
  • Tai Ji*


  • Glycation End Products, Advanced
  • Malondialdehyde
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase