Expression Profiling Using Affymetrix GeneChip Microarrays

Methods Mol Biol. 2009;509:35-46. doi: 10.1007/978-1-59745-372-1_3.


The approximately 25,000 genes in mammalian genomes can be transcribed at different levels. Measurements of gene expression for ten thousands of genes in parallel give the most comprehensive picture of steady-state levels of transcripts and is used in basic and applied research. Microarrays are the most frequently used technology for genome-wide expression profiling; from the various available microarray platforms, Affymetrix GeneChips are most frequently used for expression profiling and over 3,000 scientific publications describe results of this technology. In medical research, expression profiling by microarrays holds great promises for better understanding of diseases, identification of new therapeutic targets, and subclassification of diseases to identify individualized treatment strategies.

Publication types

  • Review

MeSH terms

  • Biotechnology / instrumentation*
  • Biotechnology / methods
  • Biotechnology / trends
  • Equipment Design
  • Equipment Failure Analysis
  • Gene Expression Profiling / instrumentation*
  • Gene Expression Profiling / methods
  • Gene Expression Profiling / trends
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Array Sequence Analysis / methods
  • Oligonucleotide Array Sequence Analysis / trends