Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

Histochem Cell Biol. 2009 May;131(5):629-42. doi: 10.1007/s00418-009-0555-7. Epub 2009 Feb 13.


In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT(1 ha). The 5-HT(1 ha) shares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT(1 ha) is relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT(1 ha) belongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT(1 ha) mRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT(1 ha) specifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT(1 ha) immunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Female
  • Ganglia, Invertebrate / metabolism*
  • Gastropoda / metabolism*
  • Gene Expression*
  • Gonads / metabolism*
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Receptor, Serotonin, 5-HT1A / genetics*
  • Sequence Alignment


  • Receptor, Serotonin, 5-HT1A