Pretreatment of reed by wet oxidation and subsequent utilization of the pretreated fibers for ethanol production

Appl Biochem Biotechnol. 2009 May;155(1-3):386-96. doi: 10.1007/s12010-009-8549-4. Epub 2009 Feb 12.


Common reed (Phragmites australis) is often recognized as a promising source of renewable energy. However, it is among the least characterized crops from the bioethanol perspective. Although one third of reed dry matter is cellulose, without pretreatment, it resists enzymatic hydrolysis like lignocelluloses usually do. In the present study, wet oxidation was investigated as the pretreatment method to enhance the enzymatic digestibility of reed cellulose to soluble sugars and thus improve the convertibility of reed to ethanol. The most effective treatment increased the digestibility of reed cellulose by cellulases more than three times compared to the untreated control. During this wet oxidation, 51.7% of the hemicellulose and 58.3% of the lignin were solubilized, whereas 87.1% of the cellulose remained in the solids. After enzymatic hydrolysis of pretreated fibers from the same treatment, the conversion of cellulose to glucose was 82.4%. Simultaneous saccharification and fermentation of pretreated solids resulted in a final ethanol concentration as high as 8.7 g/L, yielding 73% of the theoretical.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotechnology / methods
  • Cellulases / metabolism
  • Cellulose / chemistry
  • Cellulose / metabolism
  • Chromatography, High Pressure Liquid
  • Energy-Generating Resources
  • Ethanol / metabolism*
  • Fermentation / physiology*
  • Lignin / chemistry
  • Lignin / metabolism
  • Oxidation-Reduction
  • Poaceae / chemistry*
  • Poaceae / metabolism*
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism


  • Polysaccharides
  • lignocellulose
  • Ethanol
  • hemicellulose
  • Cellulose
  • Lignin
  • Cellulases