The identity of neurons influenced by oestrogen is critical for the understanding of ovarian steroid actions in the brain. The medial preoptic area (MPOA) contains one of the largest oestrogen-receptive cell populations in the rat brain and participates in several oestrogen-dependent functions, including the regulation of luteinizing hormone (LH) secretion and sexual behaviour. Using double immunostaining procedures with antibodies specific for the oestrogen receptor and neurotensin, a neuropeptide implicated in the regulation of LH secretion within this area, we found that approximately half of the neurotensin-immunoreactive neurons in the MPOA also displayed immunoreactivity for the oestrogen receptor. We estimate that oestrogen-receptive neurotensin neurons represent 5% to 12% of all oestrogen receptor-positive cells in the MPOA. Our results provide morphological evidence that neurotensin mediates oestrogen-dependent mechanisms within the brain and suggest that oestrogen may act through preoptic neurotensin neurons to aid in the generation of the LH surge.