Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction

Amrita Hazra; Abhishek Chatterjee; Tadhg P Begley

doi:10.1021/ja806752h

Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction

J Am Chem Soc. 2009 Mar 11;131(9):3225-9. doi: 10.1021/ja806752h.

Authors

Amrita Hazra¹, Abhishek Chatterjee, Tadhg P Begley

Affiliation

¹ Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.

Abstract

In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Bacillus subtilis / chemistry*
Bacillus subtilis / enzymology*
Bacillus subtilis / metabolism
Bacterial Proteins / chemistry
Bacterial Proteins / metabolism*
Biocatalysis
Carbon-Nitrogen Lyases / chemistry
Carbon-Nitrogen Lyases / metabolism*
Molecular Structure
Thiamine / biosynthesis*
Thiazoles / metabolism*

Substances

Bacterial Proteins
Thiazoles
Carbon-Nitrogen Lyases
thiamin thiazole synthase, Bacillus subtilis
Thiamine

Abstract

Publication types

MeSH terms

Substances

Grants and funding