Two pathways of synaptic vesicle retrieval revealed by single-vesicle imaging

Neuron. 2009 Feb 12;61(3):397-411. doi: 10.1016/j.neuron.2008.12.024.

Abstract

Synaptic vesicle recycling is essential for maintaining efficient synaptic transmission. Detailed dissection of single-vesicle recycling still remains a major challenge. We have developed a fluorescent pH reporter that permits us to follow the fate of individual vesicles at hippocampal synapses after exocytosis. Here we show that, during low-frequency stimulation, single-vesicle fusion leads to two distinct vesicle internalizations, instead of one, as in general perception: one by a fast endocytosis pathway ( approximately 3 s), the other by a slow endocytosis pathway (after 10 s). The exocytosed vesicular proteins are preferentially recaptured in both pathways. RNAi knockdown of clathrin inhibits both pathways. As stimulation frequency increases, the number of endocytosed vesicles begins to match antecedent exocytosis. Meanwhile, the slow endocytosis is accelerated and becomes the predominant pathway. These results reveal that two pathways of endocytosis are orchestrated during neuronal activity, establishing a highly efficient endocytosis at central synapses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Clathrin / genetics
  • Endocytosis / physiology
  • Exocytosis / physiology
  • Fluorescent Dyes
  • Hippocampus / metabolism*
  • Hippocampus / ultrastructure
  • Membrane Fusion / physiology
  • Microscopy, Fluorescence / methods
  • Neurons / metabolism*
  • Neurons / ultrastructure
  • Presynaptic Terminals / metabolism*
  • Presynaptic Terminals / ultrastructure
  • Pyridinium Compounds
  • Quaternary Ammonium Compounds
  • RNA Interference
  • Rats
  • Staining and Labeling / methods
  • Synaptic Transmission / physiology
  • Synaptic Vesicles / metabolism*
  • Synaptic Vesicles / ultrastructure
  • Time Factors

Substances

  • Clathrin
  • FM1 43
  • Fluorescent Dyes
  • Pyridinium Compounds
  • Quaternary Ammonium Compounds