Stiff coatings on compliant biofibers: the cuticle of Mytilus californianus byssal threads

Biochemistry. 2009 Mar 31;48(12):2752-9. doi: 10.1021/bi900018m.


For lasting holdfast attachment, the mussel Mytilus californianus coats its byssal threads with a protective cuticle 2-5 microm thick that is 4-6 times stiffer than the underlying collagen fibers. Although cuticle hardness (0.1 GPa) and stiffness (2 GPa) resemble those observed in related mussels, a more effective dispersion of microdamage enables M. californianus byssal threads to sustain strains to almost 120% before cuticle rupture occurs. Underlying factors for the superior damage tolerance of the byssal cuticle were explored in its microarchitecture and in the cuticular protein, mcfp-1. Cuticle microstructure was distinctly granular, with granule diameters (approximately 200 nm) only a quarter of those in M. galloprovincialis cuticle, for example. Compared with homologous proteins in related mussel species, mcfp-1 from M. californianus had a similar mass (approximately 92 kDa) and number of tandemly repeated decapeptides, and contained the same post-translational modifications, namely, trans-4-hydroxyproline, trans-2,3-cis-3,4-dihydroxyproline, and 3,4-dihydroxyphenylalanine (Dopa). The prominence of isoleucine in mcfp-1, however, distinguished it from homologues in other species. The complete protein sequence deduced from cDNAs for two related variants revealed a highly conserved consensus decapeptide PKISYPPTYK that is repeated 64 times and differs slightly from the consensus peptide (AKPSYPPTYK) of both M. galloprovincialis and M. edulis proteins.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Connective Tissue / anatomy & histology
  • Mass Spectrometry
  • Microscopy, Electron, Scanning
  • Molecular Sequence Data
  • Mytilus / anatomy & histology*
  • Proteins / chemistry


  • Proteins

Associated data

  • GENBANK/AY960601
  • GENBANK/AY960602