Five immunity-related genes previously reported to be evolving under positive selection in Drosophila melanogaster and D. simulans have been analysed across the Drosophila genus using two types of approaches, random-site and branch-site likelihood models as well as the proportion of synonymous and non-synonymous variation within and between species. Different selective pressures have been detected in the sample of genes, one showing evidence for adaptive evolution across the phylogeny of Drosophila and two showing lineage-specific positive selection. Furthermore, amino-acid sites identified as being under positive selection in the melanogaster and the virilis groups are different, suggesting that the evolution of the proteins in these two divergent groups may have been shaped by different pathogens.