Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

Lab Chip. 2009 Mar 7;9(5):692-8. doi: 10.1039/b813709a. Epub 2008 Nov 27.

Abstract

We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow. Aqueous picoliter droplets are trapped en masse and optically monitored for extended periods of time. Such an array-based approach is used to characterize droplet shrinkage, aggregation of encapsulated E. coli cells and enzymatic reactions. We also demonstrate that trapped droplets may be recovered from the microfluidic array for further processing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Biological Assay / instrumentation*
  • Dimethylpolysiloxanes / chemistry
  • Enzymes / chemistry*
  • Equipment Design
  • Escherichia coli / chemistry
  • Escherichia coli / metabolism
  • Fluorescence
  • Galactosidases / chemistry
  • Lasers
  • Luminescent Proteins / chemistry
  • Microfluidics / instrumentation*
  • Nanotechnology
  • Sulfolobus / enzymology

Substances

  • Dimethylpolysiloxanes
  • Enzymes
  • Luminescent Proteins
  • red fluorescent protein
  • baysilon
  • Galactosidases