Nevirapine Resistance in Human Immunodeficiency Virus Type 1-positive Infants Determined Using Dried Blood Spots Stored for Up to Six Years at Room Temperature

J Clin Microbiol. 2009 Apr;47(4):1209-11. doi: 10.1128/JCM.00254-09. Epub 2009 Feb 18.

Abstract

Dried blood spots that had been stored ambiently for 3 to 6 years lost approximately 1 log(10) of human immunodeficiency virus type 1 (HIV-1) RNA, but the majority could still be genotyped for resistance. Nevirapine resistance was found in 7/16 (43.5%) HIV-1-positive HIVNET 024 infants at 4 to 6 weeks, but no resistance was found at other time points.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-HIV Agents / pharmacology*
  • Blood / virology*
  • Desiccation
  • Drug Resistance, Viral*
  • Genotype
  • HIV-1 / drug effects*
  • Humans
  • Infant
  • Infant, Newborn
  • Nevirapine / pharmacology*
  • RNA, Viral / genetics*
  • Specimen Handling / methods*
  • Temperature

Substances

  • Anti-HIV Agents
  • RNA, Viral
  • Nevirapine