A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation

Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3829-34. doi: 10.1073/pnas.0808382106. Epub 2009 Feb 18.


Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Body Patterning / genetics
  • Cell Lineage
  • Conserved Sequence
  • Fetal Proteins / genetics
  • Fetal Proteins / metabolism*
  • Gastrulation / genetics
  • Gene Expression Regulation, Developmental
  • Gene Regulatory Networks*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Mesoderm / embryology*
  • Molecular Sequence Data
  • Muscles / cytology
  • Protein Binding
  • T-Box Domain Proteins / genetics
  • T-Box Domain Proteins / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Zebrafish / embryology*
  • Zebrafish / genetics*
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism


  • Fetal Proteins
  • Homeodomain Proteins
  • T-Box Domain Proteins
  • Transcription Factors
  • Zebrafish Proteins
  • noto protein, zebrafish
  • Brachyury protein

Associated data

  • GEO/GSE12331