Fibrin sealants from fresh or fresh/frozen plasma as scaffolds for in vitro articular cartilage regeneration

Tissue Eng Part A. 2009 Aug;15(8):2285-97. doi: 10.1089/ten.tea.2008.0228.


Our objective was to evaluate human CryoSeal fibrin glue derived from single units of plasma as scaffolds for articular cartilage tissue engineering. Human articular chondrocytes were encapsulated into genipin cross-linked fibrin glue derived from individual units of fresh or frozen plasma using the CryoSeal fibrin sealant (FS) system. The constructs were cultured for up to 7 weeks in vitro under low (5%) or normal (21%) oxygen. Chondrocyte viability was >90% within the fibrin gels. Hypoxia induced significant increases in collagen II and Sox9 gene expression and a significant decrease in collagen I. A significant increase in collagen II was detected in fresh plasma-derived cultures, while only collagen I was significantly increased in frozen plasma cultures. Significant increases in total glycosaminoglycan and collagen were detected in the extracellular matrix secreted by the encapsulated chondrocytes. A significant increase in compression modulus was only observed for fresh plasma-derived gels, which is likely explained by a greater amount of collagen type I detected after 7 weeks in frozen compared to fresh plasma gels. Our results indicate that CryoSeal fibrin glue derived from fresh plasma is suitable as a tissue engineering scaffold for human articular chondrocytes, and therefore should be evaluated for autologous articular cartilage regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomechanical Phenomena / drug effects
  • Blotting, Western
  • Cartilage, Articular / drug effects*
  • Cartilage, Articular / physiology*
  • Cell Death / drug effects
  • Cells, Cultured
  • Chondrocytes / cytology
  • Chondrocytes / drug effects
  • Chondrocytes / metabolism
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / metabolism
  • Fibrin Tissue Adhesive / pharmacology*
  • Gene Expression Regulation / drug effects
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
  • Integrins / metabolism
  • Oxygen / pharmacology
  • Plasma / metabolism*
  • Regeneration / drug effects*
  • Tissue Scaffolds / chemistry*


  • Fibrin Tissue Adhesive
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Integrins
  • Oxygen