Identifying protein-coding genes in genomic sequences

Genome Biol. 2009;10(1):201. doi: 10.1186/gb-2009-10-1-201. Epub 2009 Jan 30.


The vast majority of the biology of a newly sequenced genome is inferred from the set of encoded proteins. Predicting this set is therefore invariably the first step after the completion of the genome DNA sequence. Here we review the main computational pipelines used to generate the human reference protein-coding gene sets.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Base Sequence
  • Computational Biology / methods*
  • Genes
  • Genome / genetics*
  • Humans
  • Proteins / genetics*


  • Proteins