Reconstitution of chromatoid body-like particles in cultured cells: a novel approach to elucidate the mechanism of assembly and function of the chromatoid body

RNA Biol. Apr-Jun 2009;6(2):165-8. doi: 10.4161/rna.6.2.8049.

Abstract

The chromatoid body is a large perinuclear ribonucleoprotein particle of germ cells. The chromatoid body is also present in stem cells from planarians. This particle shares markers specific to protein aggresomes and P-bodies, and concentrate several RNA pathways. The assembly mechanism of chromatoid bodies is unknown, and in the absence of molecular approaches to test their function, their role as a possible center for posttranscriptional regulation of gene expression remains speculative. Recently, an RNA granule similar to the chromatoid body was fortuitously reconstituted in cultured cells by expressing a cytoplasmic isoform of the prion protein. This finding opens new avenues of investigations in the field of the still elusive chromatoid body.

Publication types

  • Review

MeSH terms

  • Animals
  • Cells, Cultured
  • Organelles / metabolism*
  • Planarians
  • RNA / genetics
  • RNA / metabolism*

Substances

  • RNA