Validation of six closely linked STRs located in the chromosome X centromere region

Int J Legal Med. 2010 Jan;124(1):83-7. doi: 10.1007/s00414-009-0328-9. Epub 2009 Feb 20.


We propose that clusters of closely linked markers, which segregate as stable haplotypes, provide a high potential to solve complex kinship cases. It is known that the X-chromosomal centromere region shows an extremely low degree of recombination. Hence, we focused our interest on the region between 56 and 64 Mb distant from the Xp telomere and considered 6 STRs which are now registered in the Genome Data Base as DXS10161, DXS10159, DXS10162, DXS10163, DXS10164, and DXS10165. All of these markers show a tetranucleotide or pentanucleotide structure and exhibit high or medium polymorphic information content. As a peculiarity, DXS10163 is a combination of a pentanucleotide STR and an 18 bp INDEL polymorphism. We report here the primer sequences, the repeat structures, the allele distributions and parameters of forensic interest for a German population sample.

Publication types

  • Validation Study

MeSH terms

  • Alleles
  • Centromere / genetics*
  • Chromosomes, Human, X / genetics*
  • DNA Fingerprinting
  • DNA Primers
  • Female
  • Gene Frequency
  • Genetics, Population*
  • Germany
  • Haplotypes
  • Humans
  • Male
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Tandem Repeat Sequences*


  • DNA Primers