beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio)

Fish Shellfish Immunol. 2009 Aug;27(2):369-73. doi: 10.1016/j.fsi.2009.02.007. Epub 2009 Feb 14.


The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5 mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5 mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5 mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1 beta but seemed to modulate IFNgamma and chemokine expression in kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Aeromonas hydrophila / physiology
  • Animals
  • Cytokines / blood
  • Fish Diseases / immunology*
  • Fish Diseases / mortality
  • Gene Expression Regulation / drug effects
  • Gram-Negative Bacterial Infections / immunology
  • Gram-Negative Bacterial Infections / mortality
  • Gram-Negative Bacterial Infections / veterinary*
  • Immunity, Innate / drug effects*
  • Kidney / cytology
  • Survival Analysis
  • Zebrafish / immunology*
  • beta-Glucans / pharmacology*


  • Adjuvants, Immunologic
  • Cytokines
  • beta-Glucans