The keyboards of multiple-user (student) and single-user (staff) computers located on a university campus were sampled to assess microbial contamination. The average number of microorganisms present on multiple-user computer keyboards was significantly greater than on single-user keyboards, and the number of keyboards harboring potential pathogens was also greater for multiple-user computers. It is recommended that regular cleaning and disinfection of computers be used to reduce the microbial load, especially for multiple-user workstations.