A simple workflow to increase MS2 identification rate by subsequent spectral library search

Proteomics. 2009 Mar;9(6):1731-6. doi: 10.1002/pmic.200800410.

Abstract

Searching a spectral library for the identification of protein MS/MS data has proven to be a fast and accurate method, while yielding a high identification rate. We investigated the potential to increase peptide discovery rate, with little increase in computational time, by constructing a workflow based on a sequence search with Phenyx followed by a library search with SpectraST. Searching a consensus library compiled from the search results of the prior Phenyx search increased the number of confidently matched spectra by up to 156%. Additionally matched spectra by SpectraST included noisy spectra, spectra representing missed cleaved peptides as well as spectra from post-translationally modified peptides.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Humans
  • Mass Spectrometry / methods*
  • Molecular Sequence Data
  • Peptide Library*
  • Proteins / analysis*
  • Proteins / chemistry
  • Software

Substances

  • Peptide Library
  • Proteins