Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets

Angew Chem Int Ed Engl. 2009;48(14):2518-21. doi: 10.1002/anie.200804326.


Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD19 / analysis
  • Biomarkers / analysis
  • Cell Line
  • Fluorescence
  • Humans
  • Membrane Proteins / analysis*
  • Microfluidic Analytical Techniques / methods*
  • Receptors, CCR5 / analysis
  • U937 Cells


  • Antigens, CD19
  • Biomarkers
  • Membrane Proteins
  • Receptors, CCR5