Sex-different and growth hormone-regulated expression of microRNA in rat liver

BMC Mol Biol. 2009 Feb 23;10:13. doi: 10.1186/1471-2199-10-13.


Background: MicroRNAs (miRNAs) are short non-coding RNAs playing an important role in post-transcriptional regulation of gene expression. We have previously shown that hepatic transcript profiles are different between males and females; that some of these differences are under the regulation of growth hormone (GH); and that mild starvation diminishes some of the differences. In this study, we tested if hepatic miRNAs are regulated in a similar manner.

Results: Using microarrays, miRNA screening was performed to identify sex-dependent miRNAs in rat liver. Out of 324 unique probes on the array, 254 were expressed in the liver and eight (3% of 254) of those were found to be different between the sexes. Among the eight putative sex-different miRNAs, only one female-predominant miRNA (miR-29b) was confirmed using quantitative real-time PCR. Furthermore, 1 week of continuous GH-treatment in male rats reduced the levels of miR-451 and miR-29b, whereas mild starvation (12 hours) raised the levels of miR-451, miR-122a and miR-29b in both sexes. The biggest effects were obtained on miR-29b with GH-treatment.

Conclusion: We conclude that hepatic miRNA levels depend on the hormonal and nutritional status of the animal and show that miR-29b is a female-predominant and GH-regulated miRNA in rat liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Nutritional Physiological Phenomena
  • Animals
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation* / drug effects
  • Growth Hormone / physiology*
  • Liver / drug effects*
  • Liver / metabolism*
  • Male
  • MicroRNAs / metabolism*
  • Rats
  • Sex Factors
  • Starvation / physiopathology


  • MicroRNAs
  • Growth Hormone