The oncoprotein LMO3 interacts with calcium- and integrin-binding protein CIB

Brain Res. 2009 Apr 10;1265:24-9. doi: 10.1016/j.brainres.2009.02.021. Epub 2009 Feb 21.

Abstract

The protein LMO3 belongs to the LIM only (LMO) group of transcriptional regulators, which act as molecular adaptors for protein-protein interactions. However, little is known about its interactive proteins and functions. Evaluating LMO3 in a yeast two-hybrid screen, we identified the calcium- and integrin-binding protein CIB as an LMO3-binding protein, which binds via the second LIM domain (LIM2) of LMO3. Cotransfection of LMO3 and CIB resulted in a shift in LMO3 protein from the nucleus to the cytoplasm. In functional assays, LMO3 induced C8 astrocyte proliferation was suppressed by the overexpression of CIB. This study demonstrates one function for LMO3 in C8 cells and suggests that one role of the CIB/LMO3 complex is to inhibit cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Astrocytes / metabolism
  • Astrocytes / physiology
  • Binding Sites / physiology
  • Calcium-Binding Proteins / metabolism*
  • Calcium-Binding Proteins / physiology
  • Carrier Proteins / metabolism
  • Carrier Proteins / physiology
  • Cell Proliferation
  • Cytoplasm / metabolism
  • DNA, Complementary
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / physiology*
  • Eye Proteins / metabolism*
  • Eye Proteins / physiology
  • Fluorescent Antibody Technique
  • Humans
  • Integrins / metabolism*
  • Integrins / physiology
  • LIM Domain Proteins
  • Membrane Glycoproteins / metabolism*
  • Membrane Glycoproteins / physiology
  • Mice
  • NIH 3T3 Cells
  • Oncogene Proteins / metabolism*
  • Oncogene Proteins / physiology*
  • Plasmids
  • Protein Binding / physiology
  • Transfection / methods
  • Two-Hybrid System Techniques

Substances

  • Adaptor Proteins, Signal Transducing
  • Calcium-Binding Proteins
  • Carrier Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Eye Proteins
  • Integrins
  • LIM Domain Proteins
  • LMO3 protein, human
  • Lim2 protein, mouse
  • Membrane Glycoproteins
  • Oncogene Proteins