Rapid Beta-Oxidation of Eicosapentaenoic Acid in Mouse Brain: An in Situ Study

Prostaglandins Leukot Essent Fatty Acids. Feb-Mar 2009;80(2-3):157-63. doi: 10.1016/j.plefa.2009.01.005. Epub 2009 Feb 23.


Analyses of brain phospholipid fatty acid profiles reveal a selective deficiency and enrichment in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), respectively. In order to account for this difference in brain fatty acid levels, we hypothesized that EPA is more rapidly beta-oxidized upon its entry into the brain. Wild-type C57BL/6 mice were perfused with either (14)C-EPA or (14)C-DHA via in situ cerebral perfusion for 40s, followed by a bicarbonate buffer to wash out the residual radiolabeled polyunsaturated fatty acid (PUFA) in the capillaries. (14)C-PUFA-perfused brains were extracted for chemical analyses of neutral lipid and phospholipid fatty acids. Based on the radioactivity in aqueous, total lipid, neutral lipid and phospholipid fractions, volume of distribution (V(D), microl/g) was calculated. The V(D) between (14)C-EPA- and (14)C-DHA-perfused samples was not statistically different for total lipid, neutral lipids or total phospholipids. However, the V(D) of (14)C-EPA in the aqueous fraction was 2.5 times higher than that of (14)C-DHA (p=0.025), suggesting a more extensive beta-oxidation than DHA. Furthermore, radiolabeled palmitoleic acid, a fatty acid that can be synthesized de novo, was detected in brain phospholipids from (14)C-EPA but not from (14)C-DHA-perfused mice suggesting that beta-oxidation products of EPA were recycled into endogenous fatty acid biosynthetic pathways. These findings suggest that low levels of EPA in brain phospholipids compared to DHA may be the result of its rapid beta-oxidation upon uptake by the brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain Chemistry
  • Cholesterol Esters / chemistry
  • Cholesterol Esters / metabolism
  • Chromatography, High Pressure Liquid
  • Docosahexaenoic Acids / analysis
  • Docosahexaenoic Acids / metabolism
  • Eicosapentaenoic Acid / analysis
  • Eicosapentaenoic Acid / metabolism*
  • Lipid Metabolism
  • Lipids / chemistry
  • Mice
  • Mice, Inbred C57BL
  • Oxidation-Reduction
  • Perfusion
  • Phospholipids / chemistry
  • Phospholipids / metabolism
  • Triglycerides / chemistry
  • Triglycerides / metabolism


  • Cholesterol Esters
  • Lipids
  • Phospholipids
  • Triglycerides
  • Docosahexaenoic Acids
  • Eicosapentaenoic Acid