Coordinated Implementation of Chikungunya Virus Reverse transcription-PCR

Emerg Infect Dis. 2009 Mar;15(3):469-71. doi: 10.3201/eid1503.081104.

Abstract

A preformulated chikungunya virus real-time reverse transcription-PCR, quality-confirmed oligonucleotides, and noninfectious virus controls were distributed by the European Network for the Diagnosis of Imported Viral Diseases. An international proficiency study with 31 participants demonstrated that ad hoc implementation of molecular diagnostics was feasible and successful.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphavirus Infections / diagnosis*
  • Alphavirus Infections / virology
  • Chikungunya virus / genetics
  • Chikungunya virus / isolation & purification*
  • Chikungunya virus / metabolism
  • Europe
  • Humans
  • Quality Control
  • RNA, Viral / analysis
  • Reagent Kits, Diagnostic*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic