Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats

Biochem Biophys Res Commun. 2009 Apr 17;381(4):602-5. doi: 10.1016/j.bbrc.2009.02.105. Epub 2009 Feb 26.


Objective: Hydrogen has been reported to selectively reduce the hydroxyl radical, the most cytotoxic of reactive oxygen species. In this study we investigated the effects of hydrogen-rich saline on the prevention of lung injury induced by intestinal ischemia/reperfusion (I/R) in rats.

Methods: Male Sprague-Dawley rats (n=30, 200-220g) were divided randomly into three experimental groups: sham operated, intestinal I/R plus saline treatment (5ml/kg, i.v.), and intestinal I/R plus hydrogen-rich saline treatment (5ml/kg, i.v.) groups. Intestinal I/R was produced by 90min of intestinal ischemia followed by a 4h of reperfusion.

Results: Hydrogen-rich saline treatment decreased the neutrophil infiltration, the lipid membrane peroxidation, NF-kappaB activation and the pro-inflammatory cytokine interleukin IL-1beta and TNF-alpha in the lung tissues compared with those in saline-treated rat.

Conclusion: Hydrogen-rich saline attenuates lung injury induced by intestinal I/R.

MeSH terms

  • Animals
  • Cell Membrane
  • Hydrogen / therapeutic use*
  • Interleukin-1beta / metabolism
  • Intestines / blood supply
  • Lipid Peroxidation
  • Lung Injury / etiology
  • Lung Injury / pathology
  • Lung Injury / prevention & control*
  • Male
  • NF-kappa B / metabolism
  • Neutrophils
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / complications
  • Sodium Chloride / therapeutic use*
  • Tumor Necrosis Factor-alpha / metabolism


  • Interleukin-1beta
  • NF-kappa B
  • Tumor Necrosis Factor-alpha
  • Sodium Chloride
  • Hydrogen