HPV infection among women in a representative rural and suburban population of the USA

Int J Gynaecol Obstet. 2009 Jun;105(3):210-4. doi: 10.1016/j.ijgo.2009.01.019. Epub 2009 Feb 28.


Objective: To study the prevalence of low-risk and high-risk HPV genotypes in a largely suburban, non-Hispanic, white female population of the USA, and to determine the positive predictive value of one-occasion HPV detection and genotyping using high-grade squamous intraepithelial lesion (HSIL) cytology as the endpoint for clinical evaluation.

Methods: HPV DNA present in liquid-based cytology specimens collected by gynecologists in private practice was amplified using nested polymerase chain reaction. HPV DNA was validated by signature DNA sequencing for accurate genotyping.

Results: Of 2633 specimens, 278 were positive for HPV DNA of any genotype. Among 255 single HPV infections, the most prevalent genotype was HPV-16 (n=50; 19.6%), followed by HPV-52 (n=24; 9.4%). Only 10 specimens, all positive for a high-risk HPV, were associated with an HSIL cytology result. Among them were 6 of the 50 specimens (12%) tested positive for HPV-16. One novel HPV-39 variant was detected in repeat testing in a patient with persistent HPV infection.

Conclusion: DNA sequencing is a useful method for increasing the specificity of HPV genotyping as an aid to follow persistent high-risk HPV infections to reduce excessive colposcopies in populations with low cancer prevalence.

MeSH terms

  • Adolescent
  • Adult
  • Age Distribution
  • Aged
  • Connecticut / epidemiology
  • DNA, Viral / analysis*
  • Female
  • Genotype
  • Humans
  • Middle Aged
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / diagnosis
  • Papillomavirus Infections / epidemiology*
  • Papillomavirus Infections / genetics
  • Polymerase Chain Reaction / methods
  • Predictive Value of Tests
  • Prevalence
  • Rural Population / statistics & numerical data
  • Sequence Analysis, DNA / methods*
  • Suburban Population / statistics & numerical data
  • Uterine Cervical Dysplasia / diagnosis
  • Uterine Cervical Dysplasia / virology
  • White People
  • Young Adult


  • DNA, Viral